User manual LEICA MM MONOPOLE DETECTION BROCHURE

[. . . ] Leica MM Monopole Detection powered by MetaMorph® Analysis Software Drop-in for Leica MM AF · Quantitation of mitotic cells with monopolar or bipolar spindles · Adaptive background correction for improved segmentation · Field and Cell-by-Cell data logging Proper formation of a bipolar spindle is vital for the segregation of chromosomes during mitosis. [. . . ] The Leica MM Monopole Detection for Leica MM AF software is designed for the quantitation of mitotic cells with monopolar or bipolar spindles where cells are labeled with a DNA stain and a second probe for microtubules. The module utilizes Adaptive Background Correction adapting the cell detection algorithm to the local intensity ranges between and within cells to provide the most robust segmentation available. This technique enables probe detection even with highly variable background fluorescence within a single image. The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Configuration for analysis 1. Specify the size range of DNA-stained cells and intensity above local background 3. Set cell classification limits based on DNA/microtubule staining correlation 5. Optionally set reporting parameters Multiple wavelength acquisition CHO-K1 cells treated with monastrol and stained with mouse anti-beta tubulin primary antibody detected with a FITC conjugated goat antimouse secondary antibody. Orange arrow shows monopole. Multi-parameter analysis The application module can generate a number of field or cell-by-cell parameters. [. . . ] Top: control, middle: monastrol, bottom: segmented image shows interphase cells (red), bipolar spindles (blue) and monopole (green). www. leica-microsystems. com [. . . ]