User manual LEICA EG 1150 H V2.2

[. . . ] Leica EG1150 H Paraffin Embedding Station Instruction Manual Leica EG1150 H V2. 2 English, Rev. Read carefully prior to operating the instrument. IMPORTANT NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. [. . . ] This includes the embedding area, forceps holder (5) and the refrigeration spot (4). A groove around the work area and several drain holes (15) are provided to allow liquid paraffin to drain rapidly. Refrigeration spot (4) The refrigeration spot is an integral part of the work area. Its position directly in front of the embedding area enables convenient working in ergonomically most favorable conditions. To orientate the samples, the mold is filled about one third with liquid paraffin. The liquid paraffin begins to solidify rapidly on the refrigeration spot. While the paraffin is semi-liquid, the sample can be oriented as required. Finally, the mold can rapidly be filled up with paraffin. While orienting the tissue, the paraffin should not become too solid, as this may cause different phases in the finished block, including fissures inside, as a result of which the block may break during sectioning. 5 3 4 3 15 4 5 Fig. 10 Forceps holder (5) The removable forceps holder under the dispenser can accommodate up to 6 forceps. The forceps holder is separately heated to approximately 70 °C. Risk of burning! Leica EG1150H ­ Paraffin Embedding Station 19 5. Operation Paraffin collection tray (8) Two indirectly-heated paraffin collection trays for excess paraffin are located under the work surface. 7 6 8 8 The paraffin collection tray must be emptied daily. To prevent contamination, do not reuse paraffin collected in this tray. If the instrument is operated without the paraffin collection trays there is risk of burning. 17 16 Mold warmer and cassette warmer (16) Depending on the preferred working direction, the two heated trays can be used for molds or cassettes (6, 7). A lid (17) is provided for each of the trays (16) to prevent loss of heat and contamination inside the tray. 11 When working with half-open lids (17), raise the temperature one level to ensure that the paraffin remains molten. Only operate the cassette and mold warmer and paraffin reservoir with lid; otherwise the set temperature cannot be maintained. 20 Instruction Manual V 2. 2, Rev B ­ 12/2009 5. Operation 21 8 Standby switch (8) After the unit has been commissioned, the standby switch should only be used if the unit is to be switched off for a longer period of time. In daily routine operation, the ON/OFF button on the control panel should be used. Pressing the ON/OFF button (20) switches the instrument into active standby mode. All displays are switched off, only the LED (21) of the ON/OFF button remains lit. If programmed procedures are to be carried out: the standby switch (8) must be switched on and the unit must be in standby mode. 12 Work area illumination (9) A halogen lamp provides homogeneous diffuse illumination of the embedding area and refrigeration spot. This gives an optimum view of the embedding process and tissue orientation. The lamp should be adjusted in a way that it is switched on and off only with the lamp button on the control panel. [. . . ] If this cannot be done by hand, do not use any hard or acute tools (e. g. Turn lamp housing upside down and lightly knock on the housing until the lamp falls out. Hold the new lamp with care (do not touch the glass bulb!) and insert in the housing. Replace cap (observing correct alignment!) and turn to the "I" to "I" position. 1 2 2 3 4 5 6 Fig. [. . . ]